Format

Send to

Choose Destination
Proc Natl Acad Sci U S A. 2006 Jun 27;103(26):10011-6. Epub 2006 Jun 16.

A humanized gnotobiotic mouse model of host-archaeal-bacterial mutualism.

Author information

1
Center for Genome Sciences, Washington University School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA.

Abstract

Our colons harbor trillions of microbes including a prominent archaeon, Methanobrevibacter smithii. To examine the contributions of Archaea to digestive health, we colonized germ-free mice with Bacteroides thetaiotaomicron, an adaptive bacterial forager of the polysaccharides that we consume, with or without M. smithii or the sulfate-reducing bacterium Desulfovibrio piger. Whole-genome transcriptional profiling of B. thetaiotaomicron, combined with mass spectrometry, revealed that, unlike D. piger, M. smithii directs B. thetaiotaomicron to focus on fermentation of dietary fructans to acetate, whereas B. thetaiotaomicron-derived formate is used by M. smithii for methanogenesis. B. thetaiotaomicron-M. smithii cocolonization produces a significant increase in host adiposity compared with monoassociated, or B. thetaiotaomicron-D. piger biassociated, animals. These findings demonstrate a link between this archaeon, prioritized bacterial utilization of polysaccharides commonly encountered in our modern diets, and host energy balance.

PMID:
16782812
PMCID:
PMC1479766
DOI:
10.1073/pnas.0602187103
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center