Format

Send to

Choose Destination
See comment in PubMed Commons below
Methods Mol Biol. 2006;326:33-60.

Tyramide signal amplification for DNA and mRNA in situ hybridization.

Author information

1
Department of Molecular Cell Biology, Research Institute Growth and Development (GROW), University of Maastricht, Maastricht, The Netherlands.

Abstract

In situ hybridization (ISH) has significantly advanced the study of gene structure and expression in cells and tissues, but its application is often limited by detection sensitivity. The introduction of signal amplification after ISH using tyramides has greatly advanced in situ detection methods that also are now applicable in routine diagnostics. In this chapter, we provide detailed step-by-step protocols for synthesis of biotinylated tyramides, multiple-target deoxyribonucleic acid-ISH on cell preparations, both DNA- and messenger ribonucleic acid (mRNA)-ISH on formalin-fixed, paraffin-embedded tissue sections, and tyramide signal amplification for signal detection.

PMID:
16780193
DOI:
10.1385/1-59745-007-3:33
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Support Center