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Cell. 2006 Jun 16;125(6):1095-109.

Targeting of aberrant mRNAs to cytoplasmic processing bodies.

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  • 1Department of Molecular and Cellular Biology, University of Arizona, Tucson, 85721, USA.

Abstract

In eukaryotes, a specialized pathway of mRNA degradation termed nonsense-mediated decay (NMD) functions in mRNA quality control by recognizing and degrading mRNAs with aberrant termination codons. We demonstrate that NMD in yeast targets premature termination codon (PTC)-containing mRNA to P-bodies. Upf1p is sufficient for targeting mRNAs to P-bodies, whereas Upf2p and Upf3p act, at least in part, downstream of P-body targeting to trigger decapping. The ATPase activity of Upf1p is required for NMD after the targeting of mRNAs to P-bodies. Moreover, Upf1p can target normal mRNAs to P-bodies but not promote their degradation. These observations lead us to propose a new model for NMD wherein two successive steps are used to distinguish normal and aberrant mRNAs.

PMID:
16777600
PMCID:
PMC1858659
DOI:
10.1016/j.cell.2006.04.037
[PubMed - indexed for MEDLINE]
Free PMC Article
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