Leuhistin, a new inhibitor of aminopeptidase M, produced by Bacillus laterosporus BMI156-14F1. I. Taxonomy, production, isolation, physico-chemical properties and biological activities

T Aoyagi; S Yoshida; N Matsuda; T Ikeda; M Hamada; T Takeuchi

doi:10.7164/antibiotics.44.573

Leuhistin, a new inhibitor of aminopeptidase M, produced by Bacillus laterosporus BMI156-14F1. I. Taxonomy, production, isolation, physico-chemical properties and biological activities

J Antibiot (Tokyo). 1991 Jun;44(6):573-8. doi: 10.7164/antibiotics.44.573.

Authors

T Aoyagi¹, S Yoshida, N Matsuda, T Ikeda, M Hamada, T Takeuchi

Affiliation

¹ Institute of Microbial Chemistry, Tokyo, Japan.

PMID: 1676997
DOI: 10.7164/antibiotics.44.573

Abstract

Leuhistin has been isolated from the culture broth of Bacillus laterosporus BMI156-14F1 as part of a program designed to find microorganism-produced inhibitors of aminopeptidase M (AP-M). It was purified by use of column chromatography on Sepabeads SP206, Amberlite IRC-50, MCI gel CHP-20P and Sephadex G-10 and then isolated as colorless needles. Leuhistin inhibits AP-M strongly and it also inhibits AP-A and AP-B weakly. It is competitive with the substrate, and the inhibition constant (Ki) was 2.3 x 10(-7) M.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acids / chemistry
Amino Acids / isolation & purification*
Amino Acids / pharmacology
Aminopeptidases / antagonists & inhibitors*
Animals
Bacillus / classification
Bacillus / metabolism*
CD13 Antigens
Imidazoles / chemistry
Imidazoles / isolation & purification*
Imidazoles / pharmacology
Mice
Molecular Weight
Oligopeptides / pharmacology
Solubility

Substances

Amino Acids
Imidazoles
Oligopeptides
probestin
leuhistin
Aminopeptidases
CD13 Antigens