Effects of various metals on DNA synthesis and lymphokines production by human peripheral blood lymphocytes in vitro

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1991;99(1-2):131-3. doi: 10.1016/0742-8413(91)90088-b.

Abstract

1. The presence of Cd2+, Ni2+, Cu2+, Mn2+ and Co2+ (10(-4)-10(-6) M) caused a dose-dependent inhibition of DNA synthesis in phytohemagglutinin (PHA) stimulated peripheral blood mononuclear cells (PBMC) from normal human donors. No inhibition of DNA synthesis was observed in the presence of Mg2+ and Al3+. 2. Spontaneous and PHA induced interleukin-1 (IL-1) was enhanced in the presence of Mn2+. 3. The release of interleukin-2 (IL-2) and soluble interleukin-2 receptors (sIL-2R) was decreased by Cd2+, Cu2+ and Mn2+. 4. Spontaneous tumor necrosis factor-alpha (TNF-alpha) production was enhanced by Co2+ and Al3+, but decreased by Cd2+. 5. gamma-Interferon (gamma-IFN) production was decreased in the presence of Cd2+ and Cu2+ and increased in the presence of Al3+.

MeSH terms

  • Aluminum / pharmacology
  • Cadmium / pharmacology
  • Cells, Cultured
  • Cobalt / pharmacology
  • Copper / pharmacology
  • DNA / biosynthesis*
  • Humans
  • Interferon-gamma / biosynthesis
  • Interleukin-1 / biosynthesis
  • Interleukin-2 / metabolism
  • Lymphocytes / drug effects
  • Lymphocytes / metabolism*
  • Lymphokines / biosynthesis*
  • Manganese / pharmacology
  • Metals / pharmacology*
  • Nickel / pharmacology
  • Phytohemagglutinins / pharmacology
  • Receptors, Interleukin-2 / metabolism
  • Tumor Necrosis Factor-alpha / biosynthesis

Substances

  • Interleukin-1
  • Interleukin-2
  • Lymphokines
  • Metals
  • Phytohemagglutinins
  • Receptors, Interleukin-2
  • Tumor Necrosis Factor-alpha
  • Cadmium
  • Cobalt
  • Manganese
  • Copper
  • Nickel
  • Interferon-gamma
  • DNA
  • Aluminum