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Free Radic Res. 2006 Jun;40(6):607-14.

Cellular and biochemical parameters of exercise-induced oxidative stress: relationship with training levels.

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Laboratory of Biology and Biochemistry of Movement, Department of Sciences of Human Movement and Sport, IUSM-University of Sport and Movement Sciences, Foro Italico piazza Lauro de Bosis 6, 00194, Rome, Italy.


To better clarify the relationship between physical activity and oxidative stress, we determined the effects of a maximal test in 18 young subjects with different training levels (six professional Athletes and 12 non-agonists (NA)). Redox homeostasis (total antioxidant activity (TAS), vitamin C and glutathione (GSH)), oxidative damage (diene conjugation and hemolysis), lymphocyte cell death and repair systems (apoptosis, micronuclei and Hsp70 expression) were evaluated. We found that agonistic training led to a chronic oxidative insult (high baseline values of oxidized glutathione (GSSG), micronuclei and hemolysis). On the contrary, NA with the lowest level of training frequency showed a well balanced profile at rest, but they were more susceptible to exercise-induced variations (GSSG/GSH and diene increased values), respect to the NA with an higher level of training. As almost all the parameters employed in this study showed inter-individual variations, the GSSG/GSH ratio remains the most sensitive and reliable marker of oxidative stress, accordingly with other data just reported in the literature.

[Indexed for MEDLINE]

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