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J Allergy Clin Immunol. 2006 Jun;117(6):1396-403. Epub 2006 Apr 27.

Biological material on inhaled coarse fraction particulate matter activates airway phagocytes in vivo in healthy volunteers.

Author information

1
Center for Environmental Medicine Asthma and Lung Biology, University of North Carolina at Chapel Hill, NC 27599-7310, USA. Neil_Alexis@med.unc.edu

Abstract

BACKGROUND:

In vitro, endotoxin on coarse fraction particulate matter (PM2.5-10) accounts for the majority of the ability of PM2.5-10 to induce cytokine responses from alveolar macrophages.

OBJECTIVE:

We examined in vivo whether inhaled PM2.5-10 from local ambient air induce inflammatory and immune responses in the airways of healthy human beings and whether biologic material on PM2.5-10 accounts for these effects.

METHODS:

On 3 separate visits, 9 healthy subjects inhaled nebulized saline (0.9%, control), PM2.5-10 collected from local ambient air that was heated to inactivate biological material (PM2.5-10-), or nonheated PM (PM2.5-10+). PM2.5-10 deposition (approximately 0.65 mg/subject) targeted the bronchial airways (confirmed by using radiolabeled aerosol), and induced sputum was obtained 2 to 3 hours postinhalation for analysis of cellular and biochemical markers of inflammation and innate immune function.

RESULTS:

Inhaled PM2.5-10+ induced elevated inflammation (% PMNs, macrophage mRNA TNF-alpha), increased eotaxin, upregulated immune surface phenotypes on macrophages (mCD14, CD11b, HLA-DR), and increased phagocytosis (monocytes) versus saline (P < .05). Biological inactivation of PM2.5-10 (PM2.5-10-) had no effect on neutrophilia but significantly (P < .05) attenuated mRNA TNF-alpha, eotaxin levels, cell surface marker responses, and phagocytosis.

CONCLUSION:

Biological components of PM2.5-10 are not necessary to induce neutrophil responses but are essential in mediating macrophage responses. The ability of PM2.5-10 to activate monocytic cells and potentially skew the airways toward an allergic phenotype by enhancing eotaxin levels may enhance responses to allergens or bacteria in individuals with allergy.

CLINICAL IMPLICATIONS:

PM2.5-10 might enhance the response of individuals with allergy to airborne bacteria.

PMID:
16751003
DOI:
10.1016/j.jaci.2006.02.030
[Indexed for MEDLINE]

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