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J Lab Clin Med. 2006 Jun;147(6):310-20.

Expression of inflammation-related genes in endothelial cells is not directly affected by microparticles from preeclamptic patients.

Author information

1
Department of Obstetrics, Academic Medical Center, Amsterdam, The Netherlands. c.a.lok@amc.uva.nl

Abstract

BACKGROUND:

Inflammation and endothelial dysfunction are prominent in preeclampsia. Microparticles (MPs) may link these processes, as MPs induce the production of pro-inflammatory cytokines by endothelial cells and cause endothelial dysfunction.

AIM:

To study changes in expression of inflammation-related genes in human endothelial cells in response to MPs from preeclamptic patients.

METHODS:

Human umbilical vein endothelial cells (HUVECs) were incubated for various time intervals in the absence or presence of isolated MP fractions from preeclamptic patients (n = 3), normotensive pregnant women (n = 3), non-pregnant controls (n = 3), and interleukin (IL)-1alpha as a positive control. Total RNA was isolated and used for multiplex ligation-dependent probe amplification (MLPA) and real-time polymerase chain reaction (PCR).

RESULTS:

IL-1alpha enhanced the expression of IL-1alpha, IL-2, IL-6, and IL-8; nuclear factor of kappa light chain enhancer in B-cells (NFkappaB)-1, NFkappaB-2, and NFkappaB-inhibitor; cyclin-dependent kinase inhibitor and monocyte chemotactic protein-1; and transiently increased tissue factor expression. RNA expression of inflammation-related genes and genes encoding adhesion receptors, however, were unaffected by any of the MP fractions tested.

CONCLUSION:

MLPA is a suitable assay to test the inflammatory status of endothelial cells, because incubation with IL-1alpha triggered substantial changes in RNA expression in endothelial cells. Taken together, it seems unlikely that MPs from preeclamptic patients induce endothelial dysfunction by directly affecting the expression of inflammation-related genes in these cells.

PMID:
16750669
DOI:
10.1016/j.lab.2006.02.004
[Indexed for MEDLINE]

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