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Microbiology. 2006 Jun;152(Pt 6):1601-7.

A plasmid-cured Chlamydia muridarum strain displays altered plaque morphology and reduced infectivity in cell culture.

Author information

1
Department of Microbiology and Immunology, UAMS, 4301 West Markham, Little Rock, AR 72205, USA. oconnellcatherine@uams.edu

Abstract

A highly conserved cryptic plasmid is present in Chlamydia trachomatis yet naturally occurring plasmid-deficient isolates are very rare. This paper describes the isolation and characterization of a plasmid-deficient strain of C. muridarum, using novobiocin as a curing agent. Plasmid-deficient derivatives of C. muridarum strain Nigg were generated at high efficiencies (4-30%). Phenotypic characterization revealed that the cured derivative was unable to accumulate glycogen within intracytoplasmic inclusions. In addition, this strain formed small plaques at a reduced efficiency compared to the wild-type parent.

PMID:
16735724
DOI:
10.1099/mic.0.28658-0
[Indexed for MEDLINE]

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