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J Antimicrob Chemother. 2006 Aug;58(2):444-8. Epub 2006 May 30.

Mechanisms of the post-antibiotic effects induced by rifampicin and gentamicin in Escherichia coli.

Author information

1
Antimicrobial Research Centre and Research Institute of Molecular and Cellular Biology, University of Leeds Leeds LS2 9JT, UK.

Abstract

OBJECTIVES:

The mechanisms by which antibiotics induce a post-antibiotic effect in susceptible bacteria are poorly understood. To explore the mechanisms more fully we examined the recovery of macromolecular synthesis in Escherichia coli during gentamicin- and rifampicin-induced post-antibiotic effects.

METHODS:

E. coli ATCC 25922 was exposed to rifampicin and to gentamicin at 5x MIC for 60 min to induce post-antibiotic effects. The antibiotics were then removed from the culture medium by washing the cells. The rates of DNA, RNA and protein synthesis during the post-antibiotic effect and recovery periods were subsequently determined by measuring the incorporation of radiolabelled uridine, thymidine and leucine into trichloroacetic acid precipitable material.

RESULTS:

Recovery of E. coli ATCC 25922 from the rifampicin-induced post-antibiotic effect coincided with the recovery of RNA and protein synthesis. Recovery from the gentamicin-induced post-antibiotic effect coincided with the recovery of protein synthesis.

CONCLUSIONS:

These data support the hypothesis that antibiotic molecules retained in the cell mediate the post-antibiotic effect by suppressing the biochemical activity of their molecular targets.

PMID:
16735423
DOI:
10.1093/jac/dkl225
[Indexed for MEDLINE]

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