A total of 149 maiden gilts (Landrace x Yorkshire) were assigned at random to one of three treatment groups at the onset of puberty. During the first and second estrus, two groups of gilts were inseminated with either 50 ml attenuated semen (n = 50) or physiological saline (n = 50). The control gilts (n = 49) remained untreated. At the third estrus, all gilts were inseminated with fresh extended semen. Intrauterine infusion with attenuated semen prior to breeding significantly increased conception rate of gilts compared with controls (82.0% vs 63.3%; P<0.05). The average interval from puberty to breeding and the age at breeding were lower in gilts treated with attenuated semen (42.75 +/- 0.89 d and 210.39 +/- 2.98 d) than control gilts (45.62 +/- 1.75 d and 218.29 +/- 3.08 d), but these differences were not significant. Litter size and weights at birth and weaning were not influenced by treatment. Results from this study indicate that presensitization to sperm antigens prior to breeding may be a useful and practical method of improving the fertility of maiden gilts.