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EMBO J. 2006 Jun 21;25(12):2768-80. Epub 2006 May 25.

Small RNA binding is a common strategy to suppress RNA silencing by several viral suppressors.

Author information

1
Agricultural Biotechnology Center, Plant Biology Institute, Gödöllõ, Hungary.

Abstract

RNA silencing is an evolutionarily conserved system that functions as an antiviral mechanism in higher plants and insects. To counteract RNA silencing, viruses express silencing suppressors that interfere with both siRNA- and microRNA-guided silencing pathways. We used comparative in vitro and in vivo approaches to analyse the molecular mechanism of suppression by three well-studied silencing suppressors. We found that silencing suppressors p19, p21 and HC-Pro each inhibit the intermediate step of RNA silencing via binding to siRNAs, although the molecular features required for duplex siRNA binding differ among the three proteins. None of the suppressors affected the activity of preassembled RISC complexes. In contrast, each suppressor uniformly inhibited the siRNA-initiated RISC assembly pathway by preventing RNA silencing initiator complex formation.

PMID:
16724105
PMCID:
PMC1500863
DOI:
10.1038/sj.emboj.7601164
[Indexed for MEDLINE]
Free PMC Article

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