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Nature. 1991 Jan 31;349(6308):429-31.

Localization of p53, retinoblastoma and host replication proteins at sites of viral replication in herpes-infected cells.

Author information

1
Imperial Cancer Research Fund, Clare Hall Laboratories, Hertfordshire, UK.

Abstract

Replication of DNA occurs at discrete sites in eukaryotic cell nuclei, where replication proteins are clustered into large complexes, or 'replicases'. Similarly, viral DNA replication is a highly structured process, notably in herpes simplex virus type-1 (HSV-1; reviewed in ref. 4) in which large globular 'replication compartments' containing the viral replication machinery exist. Replicating cellular DNA redistributes to these compartments upon HSV-1 infection. We have now used antibodies raised against several cellular proteins to detect changes in their subnuclear localization on HSV-1 infection. We found that various proteins involved in cellular DNA replication move to sites of viral DNA synthesis, whereas a selection of non-replication proteins do not. The retinoblastoma protein and p53 (the products of two putative anti-oncogenes) relocate to the same sites as known DNA replication proteins, suggesting that they may be associated with DNA replication complexes in normal, uninfected cells.

PMID:
1671528
DOI:
10.1038/349429a0
[Indexed for MEDLINE]

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