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Virology. 2006 Aug 1;351(2):489-96. Epub 2006 May 19.

Primary CCR5 only using HIV-1 isolates does not accurately represent the in vivo replicating quasi-species.

Author information

1
Wohl Virion Centre, Division of Infection and Immunity, University College London, 46 Cleveland Street, London W1T 4JF, UK.

Abstract

Most HIV-1 isolates depend on CCR5 or CXCR4 to infect target cells, and efficient use of other coreceptors is rare. We cloned HIV-1 envelopes from virus at acute infection and found that most use CCR3 efficiently. This result contradicts prevailing data, suggesting that CCR3 usage is rare. We hypothesized that direct isolation into PBMC biases selection of viruses that use CCR5 and not CCR3. We therefore compared coreceptor use of isolates obtained by PBMC coculture with envelopes cloned directly from patient blood samples, which should represent actively replicating species. Viruses derived by cloning generally used CCR3 and CCR5 with equally efficiently. In contrast, we found that viruses isolated by PBMC coculture largely, or exclusively, used CCR5. Regardless of whether CCR3 use contributes to HIV-1 transmission or pathogenesis, our results demonstrate that "primary isolates" generated by PBMC culture are unlikely to accurately represent the in vivo replicating quasi-species.

PMID:
16712896
DOI:
10.1016/j.virol.2006.04.002
[Indexed for MEDLINE]
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