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Biomed Chromatogr. 2006 Oct;20(10):1109-15.

Screening and chromatographic assessing of a novel IgG biomimetic ligand.

Author information

1
Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian 116023, People's Republic of China.

Abstract

A novel biomimetic ligand, N-benzyloxycarbonyl-l-tyrosine (N-cbz-l-Tyr), was screened by a combination method of molecular docking and immobilized receptor technique. Then, N-cbz-l-Tyr was immobilized on Sepharose CL-4B to prepare a specific affinity adsorbent for immunoglobulin G (IgG). Scatchard analysis of the binding isotherm for IgG on the adsorbent gave an association constant (K(a)) of 4.91 x 10(6) m(-1) and a theoretical maximum adsorption capacity of 17.3 mg IgG/mL gel. IgG with a purity of 98% was separated from human plasma by this new affinity adsorbent.

PMID:
16708378
DOI:
10.1002/bmc.654
[Indexed for MEDLINE]

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