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Genetics. 2006 Aug;173(4):2399-402. Epub 2006 May 15.

Exonuclease requirements for recombination of lambda-phage in recD mutants of Escherichia coli.

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1
Division of Molecular Biology, Ruder Bosković Institute, 10002 Zagreb, Croatia. dermic@irb.hr

Abstract

Recombination of lambda red gam phage in recD mutants is unaffected by inactivation of RecJ exonuclease. Since nucleases play redundant roles in E. coli, we inactivated several exonucleases in a recD mutant and discovered that 5'-3' exonuclease activity of RecJ and exonuclease VII is essential for lambda-recombination, whereas exonucleases of 3'-5' polarity are dispensable. The implications of the presented data on current models for recombination initiation in E. coli are discussed.

PMID:
16702415
PMCID:
PMC1569708
DOI:
10.1534/genetics.106.060426
[Indexed for MEDLINE]
Free PMC Article
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