(A) Metabolic parameters of 11-week-old MCP-1 Tg-B and WT mice. Data are means ± SEM (body weight, n = 5; food intake, n = 3 [WT], 4 [Tg-B]; plasma glucose, n = 9 [WT], 11 [Tg-B]; serum FFA, n = 9 [WT], 11 [Tg-B]; plasma insulin, n = 5; serum triglyceride, n = 4 [WT], 6 [Tg-B]; serum total cholesterol, n = 9 [WT], 11 [Tg-B]; serum adiponectin, n = 5). (B) Plasma glucose level during insulin (upper panel) and glucose (lower panel) tolerance tests in WT and MCP-1 Tg-B mice at 12 to 13 weeks of age. Data are mean ± SEM (n = 5). (C) Hyperinsulinemic-euglycemic clamp analysis in MCP-1 Tg-B (n = 5) and WT (n = 7) mice. Data are mean ± SEM. GIR, glucose infusion rate; Rd, rate of glucose disappearance; BHGP, basal hepatic glucose production; CHGP, hepatic glucose production during clamp. (D) Hepatic triglyceride content of 11-week-old MCP-1 Tg-B and WT mice. Data are mean ± SEM (n = 5). (E) Quantitation by Northern blot of the abundance of PEPCK, G6Pase, and SREBP-1c mRNAs in the liver of MCP-1 Tg-B and WT mice after hyperinsulinemic-euglycemic clamp analysis. Data (mean ± SEM; PEPCK and SREBP-1c, n = 5 [WT], 4 [Tg-B]; G6Pase, n = 5.) are expressed relative to the corresponding value for WT mice. *P < 0.05, **P < 0.01 versus WT.