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Sex Transm Dis. 2006 Dec;33(12):743-6.

Development of a nonhuman primate model for Trichomonas vaginalis infection.

Author information

1
Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington 98195-6460, USA. dpatton@u.washington.edu

Abstract

OBJECTIVE:

Trichomoniasis, caused by Trichomonas vaginalis, is a prevalent sexually transmitted infection associated with increased risk of HIV infection. An animal model of T. vaginalis infection would enable scientists to further investigate trichomoniasis.

STUDY DESIGN:

Seven macaques (4 test vs. 3 control) were enrolled in a 2-week pilot study. Eight additional animals participated in a 2-arm (T. vaginalis vs. sham inoculated) crossover study lasting 5 weeks before treatment. In all, 12 Macaca nemestrina monkeys were challenged with a single intravaginal inoculation of 6.6 to 7.1 x 10(5) trichomonads (ATCC 50148). Vaginal culture (InPouch TV), colposcopy, microbiology, pH, and cervical cytokines were assessed at baseline, day 2, and weekly thereafter.

RESULTS:

Ten of 12 challenged animals tested positive for trichomoniasis for 2 weeks or longer. One animal tested positive on days 2 and 7 but negative thereafter. Only one animal was not infected. Oral metronidazole treatment (35 mg/kg per day for 3 days) resolved infection in all animals. Trichomoniasis infection did not lead to shifts in vaginal microbiology or pH.

CONCLUSIONS:

A single T. vaginalis inoculation results in persistent infection in the pigtailed macaque.

[Indexed for MEDLINE]

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