Format

Send to

Choose Destination
See comment in PubMed Commons below
Clin Immunol. 2006 Jul;120(1):21-32. Epub 2006 May 4.

Multiplex mapping of CD4 T cell epitopes using class II tetramers.

Author information

1
Benaroya Research Institute at Virginia Mason, 1201 Ninth Ave. Seattle, WA 98101, USA.

Abstract

With the advent of class II tetramer technology, a tetramer-guided epitope mapping (TGEM) technique was developed for the identification of CD4+ T cell epitopes. This allowed the direct identification of epitopes recognized by the responding T cells, which were restricted to the single MHC allele of interest. However, as each individual carries multiple class II alleles, it would be advantageous to design an approach to identify CD4+ epitopes presented by different class II alleles at the same time. In the present study, a multiplex TGEM approach was developed to identify antigenic epitopes presented by multiple HLA class II alleles simultaneously. In this new approach, CD4+ T cells were stained with multiple sets of MHC class II tetramers-each labeled with a unique fluorescent label. Using this multiplex approach, novel epitopes from influenza antigens hemagglutinin and matrix protein presented by multiple class II alleles were identified in a single experimental setting.

PMID:
16677863
DOI:
10.1016/j.clim.2006.03.008
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center