Format

Send to

Choose Destination
See comment in PubMed Commons below
J Clin Virol. 2006 Jun;36(2):141-5. Epub 2006 May 4.

Clinical correlates of index values in the focus HerpeSelect ELISA for antibodies to herpes simplex virus type 2 (HSV-2).

Author information

1
University of Washington, Department of Laboratory Medicine, USA. rhoda.morrow@seattlechildrens.org

Abstract

BACKGROUND:

Clinical correlates of HerpeSelect ELISA index values are poorly understood.

OBJECTIVES:

This study was designed to determine the effects of time of infection, test variability, and antibody avidity on index values.

STUDY DESIGN:

Sera (N=313) from 81 patients with new HSV-2 infections and 236 sera from 32 patients with long-standing (median 11.3 years) HSV-2 were tested by HerpeSelect HSV-2 ELISA. High positive, low positive and negative controls were run on 42 test plates to establish test variability.

RESULTS:

Index values tended to rise after infection, peaking a median of 9-10 weeks post-infection (range 8-323 days). Of 32 patients with established HSV-2 infections, 7 (22%) had at least one low index value (>1.1 to < or =3.5), and one had a transient seroreversion event. Test variability of index values was substantially lower than inter- or intra-patient variability. Median antibody avidity was higher in sera with high versus low index values in established infections, but unrelated to index value in patients with early infections.

CONCLUSIONS:

Index values or index value changes are not absolute indicators of early versus established HSV-2 infection or solely a function of test variability. Low antibody avidity may contribute to low index values once infection is established.

PMID:
16677855
DOI:
10.1016/j.jcv.2006.02.006
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center