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Cancer Lett. 2007 Mar 8;247(1):130-6. Epub 2006 May 4.

Development of a CA125-mesothelin cell adhesion assay as a screening tool for biologics discovery.

Author information

1
Molecular Diagnostics Program, Public Health Sciences, Fred Hutchinson Cancer Research Center, University of Washington, Seattle, WA 98109-1024, USA. nscholle@fhcrc.org

Abstract

Preventing peritoneal implantation of ovarian carcinoma cells could prolong patient remission and survival. CA125 is expressed on most ovarian cancer cells and was reported to be a ligand of mesothelin, a peritoneal protein. We developed a cell adhesion assay with CA125-expresser ovarian cancer cells and human mesothelin-transfected cells and we confirmed that CA125 and mesothelin mediate cell attachment. We also showed that this assay supplies a high-throughput screening system for reagents able to block CA125/mesothelin-dependent cell attachment with a sensitive quantitative readout. We finally demonstrated that a mesothelin chimeric protein and anti-CA125 antibodies block CA125/mesothelin-dependent cell attachment.

PMID:
16677756
PMCID:
PMC2734268
DOI:
10.1016/j.canlet.2006.03.029
[Indexed for MEDLINE]
Free PMC Article

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