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J Proteome Res. 2006 May;5(5):1261-6.

Nanoporous surfaces as harvesting agents for mass spectrometric analysis of peptides in human plasma.

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1
Department of Clinical and Experimental Medicine, Magna Graecia University, 88100 Catanzaro, Italy. Gaspari@unicz.it

Abstract

Silica-based nanoporous surfaces have been developed in order to capture low molecular weight peptides from human plasma. Harvested peptides were subjected to mass spectrometric analysis by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a means of detecting and assessing the bound molecules. Peptide profiles consisting of about 70 peaks in the range 800-10,000 m/z were generated. The method could allow detection of small peptides at ng/mL concentration levels, either in standard solutions or in plasma. The same molecular cutoff effect was observed for mixtures of standard proteins and peptides incubated with silicon-based nanoporous surfaces.

PMID:
16674117
DOI:
10.1021/pr050417+
[Indexed for MEDLINE]
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