Direct serotyping of porcine rotaviruses using VP7-specific monoclonal antibodies by an enzyme immunoassay

J Med Virol. 1991 Nov;35(3):206-11. doi: 10.1002/jmv.1890350311.

Abstract

Employing a serotyping EIA test using MAbs both cell culture adapted and faecal porcine rotaviruses were classified into serotypes G3, G3/5, G4, and G5. The MAbs have confirmed and extended the serotyping results obtained using polyclonal antisera. These MAbs are therefore potential reagents for serotyping of porcine rotaviruses. Using subgroup specific MAbs serotypes G3, G3/5, and G5 were found to contain subgroup I antigens while G4 rotaviruses contained either subgroup II or subgroup I antigens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Antibody Specificity
  • Antigens, Viral*
  • Capsid / immunology
  • Capsid Proteins*
  • Gastroenteritis / microbiology
  • Gastroenteritis / veterinary
  • Immunoenzyme Techniques
  • Rotavirus / classification*
  • Rotavirus / immunology
  • Rotavirus / isolation & purification
  • Rotavirus Infections / microbiology
  • Rotavirus Infections / veterinary
  • Serotyping / methods*
  • Swine
  • Swine Diseases / microbiology

Substances

  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Antigens, Viral
  • Capsid Proteins
  • VP7 protein, Rotavirus