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Prostate. 2006 Aug 1;66(11):1203-12.

Expression of mTOR signaling pathway markers in prostate cancer progression.

Author information

1
Arizona Cancer Center, University of Arizona, 1525 N. Campbell Avenue, Tucson, AZ 85724, USA.

Abstract

BACKGROUND:

The PI3K/AKT/mTOR pathway is central to prostate cancer progression. A preliminary investigation of immuno-histochemical expression of mammalian target of rapamycin (mTOR) pathway markers was undertaken to identify patterns of expression in prostate tissue.

METHODS:

Immunohistochemistry was performed on a custom-made prostate tissue array. Mean long scores and variability of long scores for each marker were recorded for normal lumenal cells, prostate intraepithelial neoplasia (PIN), and cancer.

RESULTS:

Expression of PTEN decreased and mTOR signaling pathway markers increased in PIN and in cancer as compared to normal cells in the majority of samples. Overexpression of 4E-BP1 and p-4E-BP1 was observed in PIN and cancer. However, in cancer, the overexpression of 4E-BP1 was significantly higher than with any other marker.

DISCUSSION:

Results suggest that 4E-BP1 overexpression is strongly associated with prostate cancer, especially when combined with PTEN and mTOR expression data. Hierarchical clustering analysis utilizing PTEN, mTOR, and 4E-BP1 separated normal from cancer cell populations in most cases.

PMID:
16652388
DOI:
10.1002/pros.20410
[Indexed for MEDLINE]

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