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Proc Natl Acad Sci U S A. 2006 May 9;103(19):7402-7. Epub 2006 May 1.

Proteomic identification of oncogenic chromosomal translocation partners encoding chimeric anaplastic lymphoma kinase fusion proteins.

Author information

  • 1Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT 84132, USA. kojo.elenitobaj@path.utah.edu

Abstract

The anaplastic lymphoma kinase (ALK) on 2p23 is a tyrosine kinase that forms chimeric fusions with numerous translocation partners. We describe a mass spectrometry-based approach for the identification of ALK fusion partners. This approach accurately identified the nucleophosmin (NPM)-ALK fusion protein in an anaplastic large cell lymphoma (ALCL)-derived cell line carrying the t(2;5)(p23;q35), and the TPM3-ALK in a clinical biopsy of inflammatory myofibroblastic tumor (IMT) carrying the t(1;2)(q21;p23). This study shows the ability of mass spectrometry to identify oncogenic chimeric proteins resulting from chromosomal rearrangements. This strategy can be adapted for the identification of known and unknown translocation partners of chimeric ALK fusion proteins involved in oncogenesis.

PMID:
16651537
PMCID:
PMC1464352
DOI:
10.1073/pnas.0506514103
[PubMed - indexed for MEDLINE]
Free PMC Article
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