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Eur J Surg Oncol. 2006 Sep;32(7):707-9. Epub 2006 May 2.

Cyclo-oxygenase-2 (COX-2) mRNA expression and hormone receptor status in breast cancer.

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Breast Unit, St Bartholomew's Hospital, Barts and The London Queen Mary School of Medicine and Dentistry, West Smithfield, London EC1A 7EA, UK.



The purpose of this study was to evaluate COX-2 mRNA expression with known clinical prognostic features of breast cancer, oestrogen/progesterone receptor status, tumour size and grade.


Total RNA was extracted from 45 frozen breast tumour (invasive) and 22 normal breast tissue samples. COX-2 mRNA transcription was quantified using a real time RT-PCR assay and expressed as copy number/microg total RNA. All specimens were assessed for tumour grade, size, nodal status and presence of vascular invasion and oestrogen and progesterone receptor status.


COX-2 mRNA was detected in all samples with a median copy number of 1.15 x 10(7) for tumours and 6.5 x 10(6) for normal samples. Expression was significantly higher in oestrogen receptor negative tumours compared to the receptor positive group. There was no correlation between COX-2 mRNA levels and tumour size, grade, nodal status and presence of vascular invasion.


COX-2 mRNA expression is increased in oestrogen and progesterone receptor negative breast cancers.

[Indexed for MEDLINE]

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