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Brain Res. 1991 Nov 1;563(1-2):325-8.

Separation of alpha 1, alpha 2 and alpha 3 subunits of the GABAA-benzodiazepine receptor complex by immunoaffinity chromatography.

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1
Department of Biochemical Psychiatry, Universit├Ątsklinik f├╝r Psychiatrie, Vienna, Austria.

Abstract

Polyclonal antibodies raised to synthetic amino acid sequences of the rat gamma-aminobutyric acid (GABAA) receptor alpha 1-, alpha 2- and alpha 3-subunits selectively recognized single proteins with apparent molecular weight 51 kDa (P51), 53 kDa (P53) and 59 kDa (P59), respectively, in GABAA receptor preparations affinity purified from the brains of 5-10-day-old rats. The antibodies were coupled to Affigel 10, and the resulting immunoaffinity columns were used to isolate these proteins from affinity purified GABAA receptors.

PMID:
1664775
DOI:
10.1016/0006-8993(91)91556-g
[Indexed for MEDLINE]

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