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J Hepatol. 2006 Aug;45(2):290-8. Epub 2006 Mar 31.

Cooperative IFN-gamma production of mouse liver B cells and natural killer cells stimulated with lipopolysaccharide.

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  • 1Department of Microbiology, National Defense Medical College, Tokorozawa, Japan.



Although mouse liver contains a large population of B cells, little is known about how hepatic B cells respond to bacterial lipopolysaccharide (LPS).


The cytokine and IgM productions of hepatic B cells were compared with those of splenic B cells. The effect of LPS-treated hepatic B cells on IFN-gamma production from co-cultured NK1.1+ cells was also examined by irradiation and transwell experiments.


Hepatic B cells stimulated with LPS produced substantial amounts of IFN-gamma and IL-12 but a small amount of IgM, while splenic B cells did not produce any of these cytokines but produced a large amount of IgM. The hepatic B cells expressed surface markers similar to those on spleen B cells but expressed more C-X-C chemokine receptor 3 than spleen B cells. Notably, depletion of B220+ cells from liver MNCs (but not from spleen MNCs) greatly decreased LPS-induced IFN-gamma production. Furthermore, LPS-treated hepatic B cells stimulated liver NK1.1+ cells to produce a remarkable amount of IFN-gamma, not only through their soluble factors but also through direct cell-cell contact.


Liver B cells may play an important role in the defense against gram-negative bacterial infections by inducing IFN-gamma production from liver NK cells.

[PubMed - indexed for MEDLINE]
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