Format

Send to

Choose Destination
See comment in PubMed Commons below
Appl Environ Microbiol. 1991 Nov;57(11):3331-6.

Fractionation of cellulases from the ruminal fungus Neocallimastix frontalis EB188.

Author information

1
Department of Animal Sciences, Washington State University, Pullman 99164-6332.

Abstract

Cellulases from the ruminal fungus Neocallimastix frontalis EB188 were separated by using hydroxylapatite column chromatography. Seven carboxymethylcellulases, six avicelases, and four beta-glucosidases accounted for the majority of the activities. The separation of enzymes was confirmed by using polyacrylamide gel electrophoresis. Electrophoretic migration, analysis of hydrolysis products, and substrate specificity measurements suggested that several different cellulases were secreted in N. frontalis EB188. The possible relationship of cellulase diversity to protein glycosylation is discussed.

PMID:
1664199
PMCID:
PMC183967
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center