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J Antimicrob Chemother. 2006 Jul;58(1):183-5. Epub 2006 Apr 26.

A multiplex PCR for beta-lactamase genes of Haemophilus influenzae and description of a new blaTEM promoter variant.

Author information

1
School of Human Life Sciences, University of Tasmania Launceston, Tasmania 7250, Australia. Stephen.Tristram@utas.edu.au

Abstract

OBJECTIVES:

To establish a multiplex PCR to detect and differentiate the beta-lactamase genes of Haemophilus influenzae.

METHODS:

A collection of H. influenzae isolates with a range of different beta-lactamase genes were tested in parallel with individual PCRs and the multiplex assay developed in this study.

RESULTS:

The multiplex method was found to be superior to previous methods and was able to correctly detect and differentiate blaROB-1 and the variants of blaTEM present in the strains tested, including a previously unrecognized variant associated with a 54 bp insertion in the promoter region.

CONCLUSIONS:

The multiplex PCR will be a useful tool for the surveillance of beta-lactamase genes in H. influenzae.

PMID:
16641114
DOI:
10.1093/jac/dkl150
[Indexed for MEDLINE]

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