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Appl Microbiol Biotechnol. 2006 Aug;72(1):206-210. doi: 10.1007/s00253-006-0447-x. Epub 2006 Apr 26.

Novel blastospore-based transformation system for integration of phosphinothricin resistance and green fluorescence protein genes into Beauveria bassiana.

Author information

1
Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, 310029, People's Republic of China.
2
Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, 310029, People's Republic of China. mgfeng@zju.edu.cn.
3
Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310029, People's Republic of China. mgfeng@zju.edu.cn.

Abstract

A novel system was developed for efficient transformation of the fungal biocontrol agent Beauveria bassiana. Competent blastospores were prepared and stored in LiAc- and glycerol-inclusive suspension at -76 degrees C for sequential use in transformation. The system was successfully applied to integrating phosphinothricin resistance gene bar and enhanced green fluorescence protein gene egfp into B. bassiana via blastospore absorption of a plasmid vectoring bar and egfp. A frequency of 24 transformants per microgram of DNA was achieved. The blastospore-based transformation system has proven to be very convenient and would be highly potential for use in genetic manipulation of B. bassiana and other filamentous species.

PMID:
16639560
DOI:
10.1007/s00253-006-0447-x
[Indexed for MEDLINE]
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