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Bioresour Technol. 2007 Feb;98(3):648-53. Epub 2006 Apr 21.

Enzymatic production of biodiesel from cotton seed oil using t-butanol as a solvent.

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1
Instituto de Investigaciones para la Industria QuĂ­mica (INIQUI, CONICET), Universidad Nacional de Salta, Buenos Aires 177, 4400 Salta, Argentina. droyon@unsa.edu.ar

Abstract

The enzymatic production of biodiesel by methanolysis of cottonseed oil was studied using immobilized Candida antarctica lipase as catalyst in t-butanol solvent. Methyl ester production and triacylglycerol disappearance were followed by HPLC chromatography. It was found, using a batch system, that enzyme inhibition caused by undissolved methanol was eliminated by adding t-butanol to the reaction medium, which also gave a noticeable increase of reaction rate and ester yield. The effect of t-butanol, methanol concentration and temperature on this system was determined. A methanolysis yield of 97% was observed after 24h at 50 degrees C with a reaction mixture containing 32.5% t-butanol, 13.5% methanol, 54% oil and 0.017 g enzyme (g oil)(-1). With the same mixture, a 95% ester yield was obtained using a one step fixed bed continuous reactor with a flow rate of 9.6 mlh(-1) (g enzyme)(-1). Experiments with the continuous reactor over 500 h did not show any appreciable decrease in ester yields.

PMID:
16630719
DOI:
10.1016/j.biortech.2006.02.021
[Indexed for MEDLINE]
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