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Pancreas. 2006 Apr;32(3):306-13.

Sequence variation outside the "active" region of dog and rabbit cholecystokinin-58 results in bioactivity differences.

Author information

1
CURE: Digestive Diseases Research Center, VA GLAHS, and Digestive Diseases Division, David Geffen School of Medicine at UCLA, Los Angeles, CA 90073, USA. jreeve@ucla.edu

Abstract

OBJECTIVES:

We propose that regions outside the bioactive 7-amino acid carboxyl terminus of cholecystokinin (CCK)-58 influence its biological activity. Here we evaluate if sequence variation of the N-terminal regions of rabbit and canine CCK-58 changes their biological activities.

METHODS:

Cholecystokinin-like immunoreactivity was purified from rabbit intestinal extracts by reverse phase and ion-exchange high-performance liquid chromatography steps. The peptide was characterized by microsequence and mass spectral characterizations of the intact and tryptic peptides. Canine and rabbit CCK-58 were evaluated for their CCK1 and CCK2 receptor binding, receptor activation, and immunologic properties.

RESULTS:

The sequence of rabbit CCK-58 differs from that of canine CCK-58 in 9 of the amino terminal 40 residues. Canine CCK-58 was approximately 3-fold more potent than rabbit CCK-58 for CCK1 receptor binding and CCK2 receptor binding, but about the same potency for stimulation of amylase release from purified acinar cells. The canine peptide was 9-fold more immunoreactive than rabbit CCK-58.

CONCLUSIONS:

Canine and rabbit CCK-58 have different biological and immunologic properties that can only result from differences in their N-terminal sequences which influence the properties of their identical carboxyl termini. These results are the first direct demonstration that amino acids outside the C-terminus of CCK-58 influence CCK biological activity.

[Indexed for MEDLINE]

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