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Diagn Microbiol Infect Dis. 2006 Jul;55(3):237-40. Epub 2006 Apr 19.

Molecular diagnosis of culture-negative cerebral nocardiosis due to Nocardia abscessus.

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Laboratoire de Bactériologie, Hôpital Arnaud de Villeneuve, 34295 Montpellier Cedex 5, France.


We describe a case of culture-negative cerebral nocardiosis in a 34-year-old immunocompetent man who presented multiple cerebral abscesses. All bacteriologic cultures were negative. Nocardiosis was diagnosed by using a direct genus-specific 16S rDNA amplification method, and Nocardia abscessus was identified by hsp65 sequence analysis. The patient is alive and well on imipenem and doxycycline therapy, 14 months after onset.

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