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J Immunol Methods. 1991 Dec 15;145(1-2):105-10.

An improved immunomagnetic procedure for the isolation of highly purified human blood eosinophils.

Author information

1
Swiss Institute of Allergy and Asthma Research (SIAF), Davos-Platz.

Abstract

A simple method is described for the isolation of human peripheral blood eosinophils by an immunomagnetic procedure. Superparamagnetic particles were coupled to a monoclonal antibody against CD16, a molecule present on neutrophils but not on eosinophils. A peripheral blood granulocyte preparation, containing neutrophils and eosinophils, was incubated with these anti-CD16 particles. In the magnetic field of a permanent magnet, magnetically labelled neutrophils were then retained on columns with a ferromagnetic matrix. By this negative selection procedure, eosinophils of 99.5% purity were obtained from normal individuals and 99.6% purity from patients with eczema. A comparison was made between the immunomagnetic method and eosinophil isolation after N-formyl-methionyl-leucyl-phenyl-alanine treatment. Even in the case of individuals with very low eosinophil counts, the immunomagnetic method permits the efficient isolation of highly purified and functionally active eosinophils.

PMID:
1662676
DOI:
10.1016/0022-1759(91)90315-7
[Indexed for MEDLINE]

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