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Nat Rev Mol Cell Biol. 2006 Jun;7(6):449-56.

Visualization of molecular interactions by fluorescence complementation.

Author information

1
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109-0650, USA. kerppola@umich.edu

Abstract

The visualization of protein complexes in living cells enables the examination of protein interactions in their normal environment and the determination of their subcellular localization. The bimolecular fluorescence complementation assay has been used to visualize interactions among multiple proteins in many cell types and organisms. Modified forms of this assay have been used to visualize the competition between alternative interaction partners and the covalent modification of proteins by ubiquitin-family peptides.

PMID:
16625152
PMCID:
PMC2512262
DOI:
10.1038/nrm1929
[Indexed for MEDLINE]
Free PMC Article

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