Format

Send to

Choose Destination
Proc Natl Acad Sci U S A. 2006 May 2;103(18):6946-51. Epub 2006 Apr 18.

Genomewide gain-of-function genetic screen identifies functionally active genes in mouse embryonic stem cells.

Author information

1
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA. pritsker.moshe@mgh.harvard.edu

Abstract

Embryonic stem (ES) cells hold great promise for the future of medicine. To elucidate the molecular mechanisms that control ES cell self-renewal and differentiation, a comprehensive knowledge of the molecules involved in these processes is required. Here we describe an effective approach for genomewide identification of functionally active genes in ES cells. This approach combines genetic screens based on cDNA libraries with microarray detection methods to permit high-throughput functional analyses. We implement this strategy to identify genes whose overexpression can maintain phenotypic properties of undifferentiated mouse ES cells under differentiation-inducing conditions, specifically in the absence of leukemia inhibitory factor. The identified genes encode a variety of regulatory proteins whose function in ES cells was previously unknown. Moreover, our approach is capable of detecting genes whose overexpression promote differentiation or cell death. Overall, our studies establish a methodology for highly sensitive identification of genes that confer particular phenotypes on ES cells.

PMID:
16621925
PMCID:
PMC1458999
DOI:
10.1073/pnas.0509861103
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center