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Gene. 1991 Dec 1;108(1):115-9.

Construction of cloning vectors for Bacillus thuringiensis.

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Unité de Biochimie Microbienne, URA 1300, Centre National de la Recherche Scientifique, Institut Pasteur, Paris, France.


The replication region of the Bacillus thuringiensis plasmid, pHT1030, was treated with hydroxylamine. Various copy-number mutants were selected and subsequently used to construct shuttle vectors with multiple cloning sites. These recombinant plasmids are very stable and allowed the cloning of a delta-endotoxin-encoding gene in B. thuringiensis. Comparison between gene expression level and vector copy-number indicated that a plateau in delta-endotoxin production is reached with a copy-number of about fifteen per equivalent chromosome.

[Indexed for MEDLINE]

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