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EMBO J. 2006 May 3;25(9):1836-47. Epub 2006 Apr 13.

The molecular chaperone Hsp90 delivers precursor proteins to the chloroplast import receptor Toc64.

Author information

1
Botanik, LMU München, München, Germany.

Abstract

Precursor protein targeting toward organellar surfaces is assisted by different cytosolic chaperones. We demonstrate that the chloroplast protein translocon subunit Toc64 is the docking site for Hsp90 affiliated preproteins. Thereby, Hsp90 is recognised by the clamp type TPR domain of Toc64. The subsequent transfer of the preprotein from Toc64 to the major receptor of the Toc complex, namely Toc34, is affinity driven and nucleotide dependent. We propose that Toc64 acts as an initial docking site for Hsp90 associated precursor proteins. We outline a mechanism in which chaperones are recruited for a specific targeting event by a membrane-inserted receptor.

PMID:
16619024
PMCID:
PMC1456943
DOI:
10.1038/sj.emboj.7601091
[Indexed for MEDLINE]
Free PMC Article

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