Format

Send to

Choose Destination
Appl Radiat Isot. 2006 Aug;64(8):901-5. Epub 2006 Apr 18.

Automated, high-resolution cellular retention and uptake studies in vitro.

Author information

1
Division of Biomedical Radiation Sciences, Uppsala University, Sweden.

Abstract

This report describes an automated method for the measurements of cellular retention and uptake of radiolabeled proteins interacting with cell-surface receptors on intact cancer cells. A complete uptake and retention measurement was performed in one cell dish using a rotating radioimmunoassay (RIA) principle. Compared to common manual measurements, rotating RIA saved both labor time and reagents and provided real-time binding traces with superior time-resolution. The rotating RIA retention profiles for different interactions agreed with retention times reported in the literature.

PMID:
16618544
DOI:
10.1016/j.apradiso.2006.03.002
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center