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Nat Rev Genet. 2006 May;7(5):373-84. doi: 10.1038/nrg1836.

High-throughput RNAi screening in cultured cells: a user's guide.

Author information

1
Cenix BioScience GmbH, Tatzberg 47, Dresden 01307, Germany. echeverri@cenix-bioscience.com

Abstract

RNA interference has re-energized the field of functional genomics by enabling genome-scale loss-of-function screens in cultured cells. Looking back on the lessons that have been learned from the first wave of technology developments and applications in this exciting field, we provide both a user's guide for newcomers to the field and a detailed examination of some more complex issues, particularly concerning optimization and quality control, for more advanced users. From a discussion of cell lines, screening paradigms, reagent types and read-out methodologies, we explore in particular the complexities of designing optimal controls and normalization strategies for these challenging but extremely powerful studies.

PMID:
16607398
DOI:
10.1038/nrg1836
[Indexed for MEDLINE]
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