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Nat Genet. 2006 May;38(5):518-20. Epub 2006 Apr 9.

Mitochondrial DNA deletions are abundant and cause functional impairment in aged human substantia nigra neurons.

Author information

1
Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA.

Abstract

Using a novel single-molecule PCR approach to quantify the total burden of mitochondrial DNA (mtDNA) molecules with deletions, we show that a high proportion of individual pigmented neurons in the aged human substantia nigra contain very high levels of mtDNA deletions. Molecules with deletions are largely clonal within each neuron; that is, they originate from a single deleted mtDNA molecule that has expanded clonally. The fraction of mtDNA deletions is significantly higher in cytochrome c oxidase (COX)-deficient neurons than in COX-positive neurons, suggesting that mtDNA deletions may be directly responsible for impaired cellular respiration.

PMID:
16604072
DOI:
10.1038/ng1778
[Indexed for MEDLINE]

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