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FEBS Lett. 1991 Nov 18;293(1-2):93-6.

Mapping the site of block by tetrodotoxin and saxitoxin of sodium channel II.

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1
Max-Planck-Institut für biophysikalische Chemie, Göttingen, Germany.

Abstract

The SS2 and adjacent regions of the 4 internal repeats of sodium channel II were subjected to single mutations involving, mainly, charged amino acid residues. These sodium channel mutants, expressed in Xenopus oocytes by microinjection of cDNA-derived mRNAs, were tested for sensitivity to tetrodotoxin and saxitoxin and for single-channel conductance. The results obtained show that mutations involving 2 clusters of predominantly negatively charged residues, located at equivalent positions in the SS2 segment of the 4 repeats, strongly reduce toxin sensitivity, whereas mutations of adjacent residues exert much smaller or no effects. This suggests that the 2 clusters of residues, probably forming ring structures, take part in the extracellular mouth and/or the pore wall of the sodium channel. This view is further supported by our finding that all mutations reducing net negative charge in these amino acid clusters cause a marked decrease in single-channel conductance.

PMID:
1660007
DOI:
10.1016/0014-5793(91)81159-6
[Indexed for MEDLINE]
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