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Proteomics. 2006 May;6(9):2650-5.

Self-assembling protein arrays on DNA chips by auto-labeling fusion proteins with a single DNA address.

Author information

1
Plant Research International, Wageningen, The Netherlands. maarten.jongsma@wur.nl

Abstract

The high-throughput deposition of recombinant proteins on chips, beads or biosensor devices would be greatly facilitated by the implementation of self-assembly concepts. DNA-directed immobilization via conjugation of proteins to an oligonucleotide would be preeminently suited for this purpose. Here, we present a unique method to attach a single DNA address to proteins in one step during the purification from the E. coli lysate by fusion to human O6-alkylguanine-DNA-alkyltransferase (SNAP-tag) and the Avitag. Use of the conjugates in converting a DNA chip into a protein chip by self assembly is demonstrated.

PMID:
16596705
DOI:
10.1002/pmic.200500654
[Indexed for MEDLINE]

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