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J Bacteriol. 2006 Apr;188(8):3126-9.

An allele of gyrA prevents Salmonella enterica serovar Typhimurium from using succinate as a carbon source.

Author information

1
Department of Bacteriology, University of Wisconsin-Madison, 420 Henry Mall, Rm. 120, Madison, WI 53706, USA.

Abstract

A mutant gyrA allele resulting in an A271E substitution in the DNA gyrase protein generated a strain unable to grow on the C(4)-dicarboxylates succinate, malate, and fumarate. Bacteria harboring gyrA751 displayed decreased negative supercoiling in cells. Expression of the dctA gene, which encodes the C(4)-dicarboxylate transporter, was reduced in a gyrA751 mutant, providing the first evidence that dctA expression is supercoiling sensitive and uncovering a simple metabolic screen for lesions in gyrase that reduce negative supercoiling.

PMID:
16585773
PMCID:
PMC1446994
DOI:
10.1128/JB.188.8.3126-3129.2006
[Indexed for MEDLINE]
Free PMC Article

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