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J Mol Biol. 2006 May 12;358(4):1051-9. Epub 2006 Mar 9.

Conservation of residues involved in sugar/H(+) symport by the sucrose permease of Escherichia coli relative to lactose permease.

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1
Department of Physiology and Microbiology, Immunology and Molecular Genetics, Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095-7327, USA.

Abstract

Building a three-dimensional model of the sucrose permease of Escherichia coli (CscB) with the X-ray crystal structure lactose permease (LacY) as template reveals a similar overall fold for CscB. Moreover, despite only 28% sequence identity and a marked difference in substrate specificity, the structural organization of the residues involved in sugar-binding and H(+) translocation is conserved in CscB. Functional analyses of mutants in the homologous key residues provide strong evidence that they play a similar critical role in the mechanisms of CscB and LacY.

PMID:
16574149
PMCID:
PMC2786776
DOI:
10.1016/j.jmb.2006.02.050
[Indexed for MEDLINE]
Free PMC Article

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