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Zhongguo Zhong Yao Za Zhi. 2006 Feb;31(3):202-5.

[Simultaneous determination of resveratrol, emodin, chrysophanol, physcion, in root of Polygonam cuspidatum and its extract by HPLC].

[Article in Chinese]

Author information

1
Hunan Normal University, Changsha 410081, China.

Abstract

OBJECTIVE:

To establish a HPLC method for simultaneous determination of resveratrol, emodin, chrysophanol, physcion in root of Polygonum cuspidatum and its extract.

METHOD:

The separation was performed in a Hypersil ODS C18 column with a mobile phase of Acetonitrile-1%HAc. The flow rate was 1.0 mL x min(-1) and detection wavelength was at 287 nm.

RESULT:

The mean recovery and RSD of resveratrol, emodin, chrysophanol, physcion was 101.8%, RSD 1.7%; 96.2%, RSD 1.4%; 99.7%, RSD 1.0%; 98.60%, RSD 0.97% respectively.

CONCLUSION:

The method is accurate, simple and reliable. It can be used for quality control of P. cuspidatum and its extract.

PMID:
16572998
[Indexed for MEDLINE]

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