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J Biochem. 2006 Mar;139(3):323-7.

Application of lectin microarray to crude samples: differential glycan profiling of lec mutants.

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  • 1Nano/Bio Science Research Lab., Yokohama Technical Center, Moritex Corporation, 1-3-3 Azamino-Minami, Aoba-ku, Yokohama, Kanagawa 225-0012.

Abstract

We recently developed a novel system for lectin microarray based on the evanescent-field fluorescence-detection principle, by which even weak lectin-oligosaccharide interactions are detectable without a washing procedure. For its practical application, cell glycan analysis was performed for Chinese hamster ovary (CHO) cells and their glycan profile was compared with those of their glycosylation-defective Lec mutants. Each of the cell surface extracts gave a significantly different profile from that of the parental CHO cells in a manner reflecting denoted biosynthetic features. Hence, the developed lectin microarray system is considered to be fully applicable for differential glycan profiling of crude samples.

PMID:
16567396
DOI:
10.1093/jb/mvj070
[PubMed - indexed for MEDLINE]
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