Format

Send to

Choose Destination
See comment in PubMed Commons below
RNA. 2006 May;12(5):921-30. Epub 2006 Mar 15.

FLIPing heterokaryons to analyze nucleo-cytoplasmic shuttling of yeast proteins.

Author information

1
Wellcome Trust Centre for Cell Biology, University of Edinburgh, United Kingdom.

Abstract

Nucleo-cytoplasmic shuttling is an important feature of proteins involved in nuclear export/import of RNAs, proteins, and also large ribonucleoprotein complexes such as ribosomes. The vast amount of proteomic data available shows that many of these processes are highly dynamic. Therefore, methods are needed to reliably assess whether a protein shuttles between nucleus and cytoplasm, and the kinetics with which it exchanges. Here we describe a combination of the classical heterokaryon assay with fluorescence recovery after photobleaching (FRAP) and fluorescence loss in photobleaching (FLIP) techniques, which allows an assessment of the kinetics of protein shuttling in the yeast Saccharomyces cerevisiae.

PMID:
16540692
PMCID:
PMC1440899
DOI:
10.1261/rna.2301806
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center