Abstract
Here, we report for the first time cell-permeable and biocompatible polymeric nanoparticles consisting of a polymer conjugated to a near-infrared (NIR) fluorescence (Cy5.5)-linked effector caspase-specific peptide. The close spatial proximity of the NIR fluorochromes in polymeric nanoparticles results in an autoquenched state, but polymer nanoparticles give rise to strong NIR fluorescence signal under apoptotic cells. Thus, the smart polymeric nanoparticle developed here is an attractive probe for real-time imaging of apoptosis in single cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis / drug effects
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Apoptosis / physiology*
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Apoptosis Regulatory Proteins / pharmacology
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Biocompatible Materials / chemical synthesis
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Biocompatible Materials / chemistry*
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Biocompatible Materials / pharmacokinetics*
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Carbocyanines / chemistry
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Caspase 3
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Caspase 7
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Caspases / metabolism
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Cell Membrane Permeability
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Deoxycholic Acid / analogs & derivatives
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Deoxycholic Acid / chemistry
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HeLa Cells
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Humans
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Light
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Membrane Glycoproteins / pharmacology
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Nanostructures / chemistry*
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Oligopeptides / chemistry*
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Oligopeptides / metabolism
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Polyethyleneimine / chemistry
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Polyethyleneimine / pharmacokinetics
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Scattering, Radiation
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Spectroscopy, Near-Infrared
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Substrate Specificity
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TNF-Related Apoptosis-Inducing Ligand
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Tumor Necrosis Factor-alpha / pharmacology
Substances
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Apoptosis Regulatory Proteins
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Biocompatible Materials
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CY5.5 cyanine dye
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Carbocyanines
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Membrane Glycoproteins
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Oligopeptides
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TNF-Related Apoptosis-Inducing Ligand
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TNFSF10 protein, human
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Tumor Necrosis Factor-alpha
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Deoxycholic Acid
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Polyethyleneimine
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CASP3 protein, human
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CASP7 protein, human
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Caspase 3
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Caspase 7
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Caspases